{"id":8740,"date":"2023-08-15T11:03:52","date_gmt":"2023-08-15T15:03:52","guid":{"rendered":"https:\/\/sites.une.edu\/research\/?p=8740"},"modified":"2023-08-18T10:59:36","modified_gmt":"2023-08-18T14:59:36","slug":"carmella-st-pierre-pharmd26-is-working-with-dr-sutton-on-plastic-nanoparticles-on-an-in-vitro-model-of-the-inflamed-intestine-project-developing-skills-in-tissue-culture-sonicating-nanopar","status":"publish","type":"post","link":"https:\/\/sites.une.edu\/research\/carmella-st-pierre-pharmd26-is-working-with-dr-sutton-on-plastic-nanoparticles-on-an-in-vitro-model-of-the-inflamed-intestine-project-developing-skills-in-tissue-culture-sonicating-nanopar\/","title":{"rendered":"Carmella St. Pierre (PharmD&#8217;26) is working with Dr. Sutton on &#8220;Plastic nanoparticles on an in vitro model of the inflamed intestine&#8221; project, developing skills in tissue culture, sonicating nanoparticles and measuring their size and charge on a ZetaSizer."},"content":{"rendered":"\n<p>During the summer semester of 2023, as part of the Dean\u2019s Summer Fellowship, Carmella St. Pierre is working with Dr. Sutton on this project, developing skills in tissue culture, sonicating nanoparticles and measuring their size and charge on a ZetaSizer. The goal of this project is to add to the body of research showing the effects of microplastics on humans, animals, and on the environment, and to lend to the development of approaches to this worldwide problem. The hypothesis being considered is that nanoplastic contributes to the inflammation of the intestines.<\/p>\n\n\n\n<p>Experiments use a permeable support separating an apical and basolateral compartment. On this support, a monolayer is grown consisting of an absorptive cell line (Caco-2) and a mucous secreting cell line (HT-29). The well (basolateral compartment) contains differentiated THP-1 cells that behave like macrophages. The \u201cinflamed\u201d model is exposure to LPS &amp; IFN-\ud835\udefe, whereas the \u2018stable\u2019 model is not. The models are then exposed to TiO<sub>2<\/sub> that has been subjected to an <em>in vitro<\/em> digestion of salivary, gastric and intestinal composition. The amount of TiO<sub>2<\/sub> used has been shown not to cause inflammation. This is followed by a 72-h exposure to digested polystyrene (PS) or polymethacrylate (PMA) nanoparticles. The analysis of inflammation and cell health markers will ascertain whether the plastic nanoparticles caused an any inflammation.<\/p>\n\n\n\n<p>If the hypothesis holds, then this work could be evidence that very low levels of such food contaminants may result in poor gut health.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sites.une.edu\/research\/wp-content\/uploads\/sites\/20\/2023\/08\/Carmella-1024x768.jpeg\" alt=\"\" class=\"wp-image-8741\" style=\"width:309px;height:232px\" width=\"309\" height=\"232\" srcset=\"https:\/\/sites.une.edu\/research\/wp-content\/uploads\/sites\/20\/2023\/08\/Carmella-1024x768.jpeg 1024w, https:\/\/sites.une.edu\/research\/wp-content\/uploads\/sites\/20\/2023\/08\/Carmella-300x225.jpeg 300w, https:\/\/sites.une.edu\/research\/wp-content\/uploads\/sites\/20\/2023\/08\/Carmella-150x113.jpeg 150w, https:\/\/sites.une.edu\/research\/wp-content\/uploads\/sites\/20\/2023\/08\/Carmella-768x576.jpeg 768w, https:\/\/sites.une.edu\/research\/wp-content\/uploads\/sites\/20\/2023\/08\/Carmella-1536x1153.jpeg 1536w, https:\/\/sites.une.edu\/research\/wp-content\/uploads\/sites\/20\/2023\/08\/Carmella-2048x1537.jpeg 2048w\" sizes=\"auto, (max-width: 309px) 100vw, 309px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>During the summer semester of 2023, as part of the Dean\u2019s Summer Fellowship, Carmella St. Pierre &hellip;<\/p>\n","protected":false},"author":49,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[26],"tags":[],"post_folder":[],"class_list":["post-8740","post","type-post","status-publish","format-standard","hentry","category-student-research"],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/posts\/8740","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/users\/49"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/comments?post=8740"}],"version-history":[{"count":3,"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/posts\/8740\/revisions"}],"predecessor-version":[{"id":8757,"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/posts\/8740\/revisions\/8757"}],"wp:attachment":[{"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/media?parent=8740"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/categories?post=8740"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/tags?post=8740"},{"taxonomy":"post_folder","embeddable":true,"href":"https:\/\/sites.une.edu\/research\/wp-json\/wp\/v2\/post_folder?post=8740"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}